A-YMS
Arxula - Yeast Mineralcorticoid Screen
Detection of mineralcorticoid effects
- Comes with our test kits
- Web tool with free access
- Simple and intuitive use
- Statistical assessment of results
- Comprehensive report with graphics
- Customized report available
The A-YMS determines the CLEQ (Cortisol Equivalents) of aqueous samples.
A factorial in-house validation was performed for method validation of the A-YMS..
Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.
Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.
In the yeast genome the gene for the human mineralcorticoid receptor (NR3C2) and a reporter gene cassette have been integrated.
Mineralcorticoids bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
The reference standard for calibration and quantification is cortisol (CL). The dose-response curve corresponds to a non-linear model.
The detection is performed after reaction of the reporter enzyme phytase with the chromogenic substrate p-nitrophenyl phosphate at 405 nm.