120 €
S-YTSβ
Saccharomyces - Yeast Thyroid β Screen
Detection of thyroid receptor β mediated effects
Concerned about data analysis?
- Comes with our test kits
- Web tool with free access
- Simple and intuitive use
- Statistical assessment of results
- Comprehensive report with graphics
- Customized report available
Further documents
Available test kit format
Product Description
The S-YTSβ is an effect-based 96well assay for the the determination of thyroid receptor β mediated effects expressed as T3EQs (triiodothyronine equivalent concentration) in aqueous samples by means of the yeast Saccharomyces cerevisiae*.
*Shiizaki K, Asai S, Ebata S, Kawanishi M, Yagi T (2010) Establishment of yeast reporter assay systems to detect ligands of thyroid hormone receptors alpha and beta. Toxicol In Vitro 24:638-644
Key Characteristics
The S-YTSβ determines the T3EQ (triiodothyronine equivalents) of aqueous samples.
Validation and Standardization
A factorial in-house validation is in progress for method validation.
Data Evaluation
Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.
Support
Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.
Type of samples
Aqueous extracts
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Wastewater
For substance testing
Test Principle
Genetic Modifications
This yeast strain contains the gene for the human thyroid receptor β, the gene for the steroid receptor coactivator 1e (src1e), specific gene regulation sequences and the reporter gene lacZ.
Transactivation
Thyroid active substances bind to the thyroid receptors and the resulting homodimers trigger the transcription of the reporter gene.
Calibration
The reference standard for calibration and quantification is triiodothyronine (T3). The dose-response curve corresponds to a non-linear model.
Detection
The detection is performed after reaction of the reporter enzyme β-galactosidase with the chromogenic substrate CPRG at 580 nm.
#29303B